Effects of Cu stress on physiological, biochemical, and spectral properties of wheat at different growth stages

Zhongliang Su, Guodong Wang, Liqiang Xu, Jinheng Zhang, Xueyan Liu

Abstract


To study the mechanism of Cu toxicity on wheat, the characteristics of Cu stress in pivotal growth periods of wheat were explored by field planting methods. The results showed that at the tillering stage, the concentrations of Cu in the leaf cell fluid were significantly higher than those in the cell wall, and the Cu was primarily enriched in cell fluid. At the jointing and heading stages, the Cu concentration in the leaf cell wall was significantly higher than that in the cell fluid, and the main enrichment was transferred to the cell wall. During the above three growth stages, no Cu was discovered in leaf organelles. Further studies showed that the total soluble protein content in wheat leaves at the tillering and jointing stages showed a trend of first rising and then falling with increased Cu dosage. At the heading stage, under low and medium Cu stress, the total soluble protein content showed no remarkable change. Malondialdehyde (MDA) content at the tillering stage increased with the increase of Cu concentration in the soil, while MDA content did not change noticeably at the jointing and heading stages. At the tillering and heading stages, the low concentrations of Cu increased peroxidase (POD) activity. The POD activity decreased gradually with the increased Cu concentration. However, at the high concentrations of Cu, there was no significant difference in the activity of POD. At the jointing stage, the POD activity did not change significantly under the low Cu stress while it was evidently inhibited under high Cu stress. Based on the above studies, further analyses on the correlation between canopy spectral characteristics and the Cu accumulation at different growth stages of leaf cells were performed, and a new combined index SIPI/NDVI705 performed well in Cu content prediction. The results showed that at different growth stages, different sensitive spectral characteristic parameters should be used to predict the Cu content in leaf cells.
Keywords: Cu stress, physiology, biochemistry, heavy metal pollution, growth stage, wheat, spectrum characteristic parameter
DOI: 10.25165/j.ijabe.20191203.4403

Citation: Su Z L, Wang G D, Xu L Q, Zhang J H, Liu X Y. Effects of Cu stress on physiological, biochemical, and spectral properties of wheat at different growth stages. Int J Agric & Biol Eng, 2019; 12(3): 147–153.

Keywords


Cu stress, physiology, biochemistry, heavy metal pollution, growth stage, wheat, spectrum characteristic parameter

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References


Paolo Z, Saša K. Two aquatic macrophytes as bioindicators for medium-high Cu concentrations in freshwaters. Giornale Botanico Italiano, 2011; 145(2): 503–506.

Moreno J E, Gimeno H, Gamarra R, Esteban E. Evidence of a new Hg-tolerant ecotype of Rumex induratus from Almadén (Ciudad Real, Spain). Giornale Botanico Italiano, 2014; 148(1): 58–63.

Huang J F, Blackbum G A. Optimizing predictive models for leaf chlorophyll concentration based on continuous wavelet analysis of hyperspectral data. International Journal of Remote Sensing, 2011; 32(24): 9375–9396.

Jiang J, Gu X, Song R, Wang X, Yang L. Microcystin-LR induced oxidative stress and ultrastructural alterations in mesophyll cells of submerged macrophyte Vallisneria natans (Lour.) Hara. Journal of Hazardous Materials, 2011; 190(1-3): 188–196.

Bailey-Serres J, Mittler R. The roles of reactive oxygen species in plant cells. Plant Physiology, 2006; 141(2): 311.

Halliwell B. Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiology, 2006; 141(2): 312–322.

Dunagan S C, Gilmore M S, Varekamp J C. Effects of mercury on visible/near-infrared reflectance spectra of mustard spinach plants (Brassica rapa P.). Environmental Pollution, 2007; 148(1): 301–311.

Clevers J G P W, Kooistra L, Salas E A L. Study of heavy metal contamination in river floodplains using the red-edge position in spectroscopic data. International Journal of Remote Sensing, 2004; 25(19): 3883–3895.

Liu F, Liu X N, Ding C, Wu L. The dynamic simulation of rice growth parameters under cadmium stress with the assimilation of multi-period spectral indices and crop model. Field Crops Research, 2015; 183: 225–234.

Khoiri M, Chang-Yu O U, Teng F C. Reduction in germination and seedling growth of Thespesia populnea L. caused by lead and cadmium treatments. Pakistan Journal of Botany, 2008; 40(6): 2419–2426.

Zhao S L, Liu Q, Qi Y T, Duo L. Responses of root growth and protective enzymes to Cu stress in turfgrass. Acta Biologica Cracoviensia, 2010; 52(2): 7–11.

Guo T R. Photosynthesis and antioxidant capacity in barley seedlings under the combined toxicity of cadmium and aluminum. Advanced Materials Research, 2013; 610-613: 467–471.

Liang H, Liu X. Hyperspectral analysis of leaf Cu accumulation in agronomic crop based on artificial neural network. International Workshop on Earth Observation and Remote Sensing Applications. IEEE, 2008; pp.1–6.

Wang H, Zeng L S, Sun Y H, Zhang J H, Guo Q Z, Sun F L, et al. Wheat canopy spectral reflectance feature response to heavy metal copper and zinc stress. Transactions of the CSAE, 2017; 33(2): 171–176. (in Chinese)

Qiu R L, Thangavel P, Hu P J, Senthilkumar P, Ying R R, Tang Y T. Interaction of cadmium and zinc on accumulation and sub-cellular distribution in leaves of hyperaccumulator Potentilla griffithii. Journal of Hazardous Materials, 2011; 186(3): 1425–1430.

Rao M V, Paliyath G, Ormrod D P. Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiology, 1996; 110(1): 125–136.

Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976; 72: 248–254.

Yang Y L, Zhang Y Y, Wei X L, You J, Wang W R, Lu J, et al. Comparative antioxidative responses and proline metabolism in two wheat cultivars under short term lead stress. Ecotoxicology & Environmental Safety, 2011; 74(4): 733–740.

Tong Q X, Zhang B, Zheng L F. Hyperspectral remote sensing and it's multidisciplinary applacation. Beijing: Publishing House of Electronics Industry, 2006; 42. (in Chinese)

Zhang Y X, Chai T Y, Burkard G. Research advances on the mechanisms of heavy metal tolerance in plants. Acta Bota nica Sinica, 1999; 41(5): 453–457. (in Chinese)

Gong Z, Zhao Y, Zhao W. Estimation model for plant leaf chlorophyll content based on the spectral index content. Acta Ecologica Sinica, 2014; 34(20): 9–15. (in Chinese)

Koca H, Ozdemir F, Turkan I. Effect of salt stress on lipid peroxidation

and superoxide dismutase and peroxidase activities of Lycopersicon esculentum and L. pennellii. Biologia Plantarum, 2006; 50(4): 745–748.

Zhang S, Xiao X, Jia H X, Sun Z L, Bai X L. Subcellular distribution of cu in jointing wheat. Environmental Science & Technology, 2011; 34(5): 56–60. (in Chinese)

Guan G, Song X U. The regularity of distribution, change and migration of heavy metals in soil-rice plant system. Ecology & Environment, 2006; 15(2): 315–318. (in Chinese)

Josep P, Iolanda F, John A G. Assessment of photosynthetic radiation-use efficiency with spectral reflectance. New Phytol., 1995; 131(3): 291–296. (in Chinese)

He L, Fan B W. Heavy metals in marine environment and their effects on marine organisms. Guangzhou Chemistry, 2006; 31(3): 63–69. (in Chinese)

Celina M L, Claudio A G, Victorio S T. Oxidative damage caused by an excess of Cu in oat leaves. Plant & Cell Physiology, 1994; 35: 11–15. (in Chinese)

Ge C L, Yang X Y, Sun J H, Wang Z G, Luo S S, Ma F, et al. DNA damage caused by heavy metal stress in rice and wheat seedlings. Journal of Plant Physiology and Molecular Biology, 2002; 28(6): 419–424. (in Chinese)

Xu, X Y, Shi G X, Wang J, Zhang L L, Kang Y N. Cu-induced oxidative stress in Alternanthera philoxeroides callus. Plant Cell Tissue & Organ Culture, 2011; 106(2): 243–251. (in Chinese)

Ute K, Janet D C H, John M C, Alan J M B, J A C S. Free histidine as a metal chelator in plants that accumulate nickel. Nature, 1996; 379: 635–638. (in Chinese)

Xue P Y, Li G X, Zhao Q L. Mechanisms of Cu uptake by submerged plant Hydrilla verticillata (L. f.) Royle and Myrio-phyllum spicatum L. Environmental Science, 2014; 35(5): 1878–1883. (in Chinese)

Zhang C M, Zhang C F, Zhu Q Q, Jia Y X, Luo X, Zhang S R, et al. Physiological response and subcellular distribution of Cu in Lantana camara L. Journal of Agro-Environment Science, 2016; 35(1): 21–28. (in Chinese)

Ren Y F, He J Y, Liu C, Luo X L, Huang T X. Effects of Cd stress on seedling growth and activities in antioxidant enzymes of lettuce. Ecology and Environmental Sciences, 2009; 18(2): 494–497. (in Chinese)

Xiong C H, Lu Y E, Ouyang B, Xia J H, Zhang Y Y, Li H X. Effects of Pb or Cd stress on the development, growth and yield of taro. Journal of Agricultural Resources and Environment, 2016; 33(5): 1–8. (in Chinese)

Ajayan K V, Selvaraju M. Heavy metal induced antioxidant defense system of green microalgae and its effective role in phycoremediation of tannery effluent. Pakistan Journal of Biological Sciences, 2012; 15(22): 1056–1062.

Benedict H M, Swidler R. Nondestructive method for estimating Chlorophyll content of leaves. Science, 1961; 133(3469): 2015–2016.

Gu Y W, Li S, Gao W, Wei H. Hyperspectral estimation of the cadmium content in leaves of Brassica rapa chinesis based on the spectral parameters. Acta Ecologica Sinica, 2015; 35(13): 4445–4453. (in Chinese)




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